Protein Purification Reagents and Kits
CUBE BIOTECH PureCube Fe-NTA MagBeads; 5 ml
PureCube Fe-NTA MagBeads were developed for the enrichment of phosphorylated proteins the affinity matrix is based on the spherical magnetic agarose beads, consisting og 6% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. The magnetic beads are very homogeneous in size with a medium particle diameter of 30um, yielding a high degree of reproducibility between individual purificaiton runs.
Bio Rad Laboratories ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, Combinatorial peptide ligand library, 500 μL bead slurry per column, 20 μL settled bead volume
ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, Combinatorial peptide ligand library, 500 μL bead slurry per column, 20 μL settled bead volume
Epigentek Inc EpiQuik Nuclear Extraction Kit (100 assays)
For isolation of nuclear proteins from cells or tissues while keeping enzymatic activity intact
Molecular Dimensions STRUCTURE SCREEN I + II HT-96
Protein Crystallography. Structure Screen 1 + 2 HT-96 (96 x 1.1 mL) is formulated for the crystallization of proteins, peptides, nucleic acids and water soluble small molecules.
STEMCELL Technologies EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
Genscript Corporation Glutathione Resin
Glutathione Resin (Cat. No. L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. Table 1 lists the main characteristics of Glutathione Resin.
Aat Bioquest HIS Lite™ OG488-Tris NTA-Ni Complex, green fluorescent probe, 100 μg, suitable for polyhistidine-tagged proteins, fluorescent detection
HIS Lite™ OG488-Tris NTA-Ni Complex, green fluorescent probe, 100 μg, suitable for polyhistidine-tagged proteins, fluorescent detection
Gentarget HTERT EF1A GFP-PURO LENTIVIRUS
Pre-made Lentivirus expressing Human Telomerase Reverse Transcriptase /hTERT NM_198253.2/ under enhanced EF1a promoter. A GFP-Puromycin fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic or via cell sorting by GFP fluorescent signal. hTERT is widely used for cell immortalization used alone or together with other cell immortalization factors like SV40 large T-antigen.
Hampton Research SOLUBILITY & STABILITY SCREEN
HR2-072/Solubility & Stability Screen - 0.5 ml, Deep Well block format. Please note items from this supplier are non-returnable.
STEMCELL Technologies EasySep™ Release Human CD4 Positive Selection Kit, For processing: 1 x 10^9 cells
The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Surmodics Inc StabilGuard Immunoassay Stabilizer, 50mL
StabilGuard™ Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefit of the protein free formulation is the elimination of interference and cross reactivity within immunoassay applications.